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SARS-CoV-2 Spike Protein (D614G, Super Stable Trimer) (His tag,AVI tag,Biotin)

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
Catalog No. ABIN6953300
  • Target See all SARS-CoV-2 Spike Proteins
    SARS-CoV-2 Spike
    Protein Type
    Recombinant
    Protein Characteristics
    D614G, Super Stable Trimer
    Origin
    • 223
    • 37
    • 9
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    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
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    • 1
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    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 198
    • 7
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike protein is labelled with His tag,AVI tag,Biotin.
    Application
    ELISA
    Purpose
    Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)
    Sequence
    AA 16-1213
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1(D614G) and D614G mutation, which has become increasingly common in SARS-CoV-2 viruses from around the world .
    The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus.
    Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
    It is the biotinlynated form of SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified) SPN-C52H3).
    Purity
    > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product SARS-CoV-2 Spike Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS
    Storage
    -20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower.
    This product is stable after storage at:
    -20°C to -70°C for 12 months in lyophilized state
    70°C for 3 months under sterile conditions after reconstitution.
    Expiry Date
    12 months
  • Target
    SARS-CoV-2 Spike
    Abstract
    SARS-CoV-2 Spike Products
    Synonyms
    E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
    Target Type
    Viral Protein
    Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    139.7 kDa
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