SARS-CoV-2 Spike Protein (Trimer) (rho-1D4 tag)
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- Target See all SARS-CoV-2 Spike Proteins
- SARS-CoV-2 Spike
- Protein Type
- Recombinant
- Protein Characteristics
- Trimer
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Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Purpose
- Trimeric, full length Cov-2 spike protein for assay development (""Antibody tests"")
- Specificity
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- single span transmembrane membrane protein (aa 1-1273)
- Furin cleavage site ""RRAR"" mutated to ""GSAS""
- trimerization (3 x 142 kDa) shown on native PAGE
- expressed in Expi293™ cells
- C-terminal Rho1D4 tag for affinity purification
- Solubilization and stabilization in LMNG detergent
- 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent
- Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Full length SARS Cov-2 spike protein expressed in Expi293™ cells to assure native state glycosylation.
- Purification by a multi-step, protein-specific protocol to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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The protein is purified from the cleared cell lysate using Rho1D4 capture materials. Eluate fractions are analyzed by SDS-PAGE.
Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot. - Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Protein is endotoxin-free.
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (B.1.351 - beta) protein (rho-1D4 tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (B.1.1.7 - alpha) protein (rho-1D4 tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha Host: HEK-293 Cells > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (P.1 - gamma) protein (rho-1D4 tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (B.1.617.1 - kappa) protein (rho-1D4 tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa Host: HEK-293 Cells Recombinant > 98% as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker) - Restrictions
- For Research Use only
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- Buffer
- 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Products
- Synonyms
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Target Type
- Viral Protein
- Background
- The spike glycoprotein exists as a homotrimeric fusion protein. Each of the trimers contains 66 glycosylation sites for host-derived N-linked glycans. Accordingly, expression of this primary target for SARS-CoV-2 vaccine development in an appropriate, human expression system is of utmost importance. Prior to ACE2 binding, each monomer in the prefusion complex contains an S1 ectodomain including the receptor binding domain (RBD) and an S2 endodomain harboring a transmembrane domain. In the predominant state of the trimer, one of the RBDs is in an “up” position whereas the other two are in a “down” position. Interaction of S-protein and ACE2 only takes place with the RBD in the “up” position. Receptor binding triggers a structural change that leads to separation of the S1 and S2 subunits.
- Molecular Weight
- 3 x 142 kDa
- Gene ID
- 43740568
- UniProt
- P0DTC2
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