For best experience we recommend to activate Javascript in your browser.

Mesothelin antibody (Extracellular Domain)

Name: Mesothelin antibody (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN233753
Price: 577.5 USD
Availability: InStock

MSLN Reactivity: Human WB, IHC Host: Mouse Monoclonal MN-1 unconjugated

Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods.

IHC

Western blotting using anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.

Mesothelin expression in mesothelioma monolayers and spheroids.

FACS

3 images

(8 references)

Catalog No. ABIN233753

Datasheet as PDF

Target See all Mesothelin (MSLN) Antibodies

Mesothelin (MSLN)
Binding Specificity
16

9

6

6

5

5

5

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Extracellular Domain
Reactivity
88

24

5

2

2

2

1

1

1
Human
Host
75

24

4

1

1
Mouse
Compatible Secondaries
Clonality
74

29

2
Monoclonal
Conjugate
67

12

8

6

1

1

1

1

1

1

1

1

1

1

1

1
This Mesothelin antibody is un-conjugated
Application
66

51

24

21

12

11

8

5

4

4

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

Characteristics

Concentration Definition: by UV absorbance at 280 nm

Immunogen

This antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the of human mesothelin.
Immunogentype:Recombinant

Clone

MN-1

Isotype

IgG

Top Product

Discover our top product MSLN Primary Antibody
anti-Mesothelin (MSLN) (AA 273-407) antibody
MSLN Reactivity: Human IHC (f) Host: Mouse Monoclonal MSLN-2131 unconjugated

anti-Mesothelin (MSLN) (AA 101-200) antibody
MSLN Reactivity: Human ELISA, WB, FACS, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Mesothelin (MSLN) (AA 37-286) antibody
MSLN Reactivity: Human, Mouse ELISA, WB, FACS Host: Mouse Monoclonal 5C11A4 unconjugated

anti-Mesothelin (MSLN) (AA 296-580) antibody
MSLN Reactivity: Human ELISA, FACS Host: Rabbit Monoclonal DM71 unconjugated Recombinant Antibody

anti-Mesothelin (MSLN) (AA 273-407) antibody
MSLN Reactivity: Human IHC (f) Host: Mouse Monoclonal unconjugated

anti-Mesothelin (MSLN) (AA 37-598) antibody
MSLN Reactivity: Human ELISA, IHC, IP, IF Host: Rabbit Polyclonal unconjugated

anti-Mesothelin (MSLN) (AA 296-606) antibody
MSLN Reactivity: Human, Mouse ELISA, WB Host: Mouse Monoclonal 7E6A6 unconjugated

anti-Mesothelin (MSLN) (Extracellular), (Extracellular Domain) antibody
MSLN Reactivity: Human WB, IHC Host: Mouse Monoclonal MB-G10 unconjugated

anti-Mesothelin (MSLN) (AA 273-407) antibody (Biotin)
MSLN Reactivity: Human IHC (f) Host: Mouse Monoclonal Biotin

anti-Mesothelin (MSLN) (AA 1-62) antibody
MSLN Reactivity: Human ELISA, WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C
Zhang, Khanna, Jiang, Alewine, Miettinen, Pastan, Hassan: "Efficacy of Anti-mesothelin Immunotoxin RG7787 plus Nab-Paclitaxel against Mesothelioma Patient-Derived Xenografts and Mesothelin as a Biomarker of Tumor Response." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 23, Issue 6, pp. 1564-1574, (2018) (PubMed).

Oehl, Kresoja-Rakic, Opitz, Vrugt, Weder, Stahel, Wild, Felley-Bosco: "Live-Cell Mesothelioma Biobank to Explore Mechanisms of Tumor Progression." in: Frontiers in oncology, Vol. 8, pp. 40, (2018) (PubMed).

Khanna, Thomas, Abate-Daga, Zhang, Morrow, Steinberg, Orlandi, Ferroni, Schlom, Guadagni, Hassan: "Malignant Mesothelioma Effusions Are Infiltrated by CD3+ T Cells Highly Expressing PD-L1 and the PD-L1+ Tumor Cells within These Effusions Are Susceptible to ADCC by the Anti-PD-L1 Antibody Avelumab." in: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, Vol. 11, Issue 11, pp. 1993-2005, (2017) (PubMed).

Awuah, Bera, Folivi, Chertov, Pastan: "Reduced Shedding of Surface Mesothelin Improves Efficacy of Mesothelin-Targeting Recombinant Immunotoxins." in: Molecular cancer therapeutics, Vol. 15, Issue 7, pp. 1648-55, (2017) (PubMed).

Xiang, Phung, Feng, Nagashima, Zhang, Broaddus, Hassan, Fitzgerald, Ho: "The development and characterization of a human mesothelioma in vitro 3D model to investigate immunotoxin therapy." in: PLoS ONE, Vol. 6, Issue 1, pp. e14640, (2011) (PubMed).

Feng, Zhang, Anver, Hassan, Ho: "In vivo imaging of human malignant mesothelioma grown orthotopically in the peritoneal cavity of nude mice." in: Journal of Cancer, Vol. 2, pp. 123-31, (2011) (PubMed).

Ho, Bera, Willingham, Onda, Hassan, FitzGerald, Pastan: "Mesothelin expression in human lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 5, pp. 1571-5, (2007) (PubMed).

Onda, Willingham, Nagata, Bera, Beers, Ho, Hassan, Kreitman, Pastan: "New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 11, Issue 16, pp. 5840-6, (2005) (PubMed).
Target

Mesothelin (MSLN)

Alternative Name

Mesothelin (MSLN Products)

Synonyms

MPF antibody, SMRP antibody, MSLN antibody, mesothelin antibody, MSLN antibody, Msln antibody, LOC611363 antibody, LOC100524016 antibody

Background

Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Synonyms: Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen

Gene ID

10232

NCBI Accession

NP_005814

UniProt

Q13421

Pathways

EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process