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NLRP3 antibody (Pyrin Domain)

Name: NLRP3 antibody (Pyrin Domain)
Brand: antibodies-online
SKU: ABIN1169100
Price: 529.38 USD
Availability: InStock

NLRP3 Reactivity: Human, Mouse WB, IHC, IF, IP Host: Mouse Monoclonal Cryo-2 unconjugated

Mouse C2C12 myoblasts or the differentiated myocytes using horse serum were treated with LPS (1 μg/mL) or IFN-γ (100 ng/mL) for 24 to 72 h.

Endogenous co-immunoprecipitation of ASC and NLRP3 in WT or Sarm1-/- iBMDMs following NLRP3 inflammasome activation.

Co-IP

Immunofluorescence staining of mouse brain tissues

Co-IP

Whole cell lysates were analyzed by western blotting.

5 images

(60 references)

Catalog No. ABIN1169100

Datasheet as PDF

Key Features
- NLRP3 antibody for reliable recognition of mouse and human NLRP3/NALP3
- This un-conjugated monoclonal antibody has 60 PubMed citations
Target See all NLRP3 Antibodies

NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
Binding Specificity
16

9

8

7

7

6

5

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 1-93, Pyrin Domain
Reactivity
91

56

38

23

4

2

1

1

1
Human, Mouse
Host
88

10

2

1
Mouse
Compatible Secondaries
Clonality
86

15
Monoclonal
Conjugate
44

9

8

7

4

3

3

3

3

3

3

2

2

2

2

2

1
This NLRP3 antibody is un-conjugated
Application
63

40

22

13

13

10

9

8

7

6

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity

Recognizes mouse and human NLRP3/NALP3.

Cross-Reactivity

Human, Mouse (Murine)

Purification

Purified from concentrated hybridoma tissue culture supernatant.

Purity

>95 % (SDS-PAGE)

Immunogen

Recombinant mouse NLRP3/NALP3 (pyrin domain) (aa 1-93).

Clone

Cryo-2

Isotype

IgG2b
anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 15-120) antibody
NLRP3 Reactivity: Human, Mouse, Rat, Dog WB, ELISA, IHC (p), FACS, IF (p), ICC, IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 671-1033) antibody
NLRP3 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (C-Term) antibody
Verified NLRP3 Reactivity: Human ELISA, IHC, FACS, IF Host: Goat Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (C-Term) antibody
NLRP3 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (N-Term) antibody
NLRP3 Reactivity: Human, Mouse, Rat WB, IHC (p), FACS, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 65-91), (N-Term) antibody
NLRP3 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB22404 unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 30-102) antibody
NLRP3 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-100) antibody
NLRP3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3B1 unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 120-170) antibody
NLRP3 Reactivity: Human, Mouse WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 21-194) antibody
NLRP3 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date

12 months
Zhu, Tang: "LncRNA Gm14205 induces astrocytic NLRP3 inflammasome activation via inhibiting oxytocin receptor in postpartum depression." in: Bioscience reports, Vol. 40, Issue 8, (2020) (PubMed).

Wong, Lewis, Doherty, Shi, Cashikar, Amelianchik, Tymchuk, Sullivan, Qian, Covey, Petsko, Holtzman, Paul, Luo: "25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner." in: Journal of neuroinflammation, Vol. 17, Issue 1, pp. 192, (2020) (PubMed).

Hoyle, Redondo-Castro, Cook, Tzeng, Allan, Brough, Lemarchand: "Hallmarks of NLRP3 inflammasome activation are observed in organotypic hippocampal slice culture." in: Immunology, Vol. 161, Issue 1, pp. 39-52, (2020) (PubMed).

Shrivastava, Visoso-Carvajal, Garcia-Cordero, Leon-Juarez, Chavez-Munguia, Lopez, Nava, Villegas-Sepulveda, Cedillo-Barron: "Dengue Virus Serotype 2 and Its Non-Structural Proteins 2A and 2B Activate NLRP3 Inflammasome." in: Frontiers in immunology, Vol. 11, pp. 352, (2020) (PubMed).

Wang, Hu, Wu, Feng, Li, Liu, Wang, Chen, Tian, Xiao, Zhang, Shereen, Chen, Pan, Wan, Wu, Wu: "STING promotes NLRP3 localization in ER and facilitates NLRP3 deubiquitination to activate the inflammasome upon HSV-1 infection." in: PLoS pathogens, Vol. 16, Issue 3, pp. e1008335, (2020) (PubMed).

Lai, Hsu, Lee, Chiang: "Microtubule-Mediated NLRP3 Inflammasome Activation Is Independent of Microtubule-Associated Innate Immune Factor GEF-H1 in Murine Macrophages." in: International journal of molecular sciences, Vol. 21, Issue 4, (2020) (PubMed).

Wooff, Fernando, Wong, Dietrich, Aggio-Bruce, Chu-Tan, Robertson, Doyle, Man, Natoli: "Caspase-1-dependent inflammasomes mediate photoreceptor cell death in photo-oxidative damage-induced retinal degeneration." in: Scientific reports, Vol. 10, Issue 1, pp. 2263, (2020) (PubMed).

Svensson, Andersson, Manouchehrian, Yang, Deierborg: "Voluntary running does not reduce neuroinflammation or improve non-cognitive behavior in the 5xFAD mouse model of Alzheimer's disease." in: Scientific reports, Vol. 10, Issue 1, pp. 1346, (2020) (PubMed).

Cai, Ge, Mennone, Hoque, Ouyang, Boyer: "Inflammasome Is Activated in the Liver of Cholestatic Patients and Aggravates Hepatic Injury in Bile Duct-Ligated Mouse." in: Cellular and molecular gastroenterology and hepatology, Vol. 9, Issue 4, pp. 679-688, (2020) (PubMed).

Dionísio, Oliveira, Gaspar, Gama, Castro-Caldas, Amaral, Rodrigues: "Ablation of RIP3 protects from dopaminergic neurodegeneration in experimental Parkinson's disease." in: Cell death & disease, Vol. 10, Issue 11, pp. 840, (2020) (PubMed).

Du, Lu, Wang, Ding, Wu, Hu: "The pore-forming subunit Kir6.1 of the K-ATP channel negatively regulates the NLRP3 inflammasome to control insulin resistance by interacting with NLRP3." in: Experimental & molecular medicine, Vol. 51, Issue 8, pp. 1-13, (2020) (PubMed).

Hoss, Mueller, Rojas Ringeling, Rodriguez-Alcazar, Brinkschulte, Seifert, Stahl, Broderick, Putnam, Kolodner, Canzar, Geyer, Hoffman, Latz: "Alternative splicing regulates stochastic NLRP3 activity." in: Nature communications, Vol. 10, Issue 1, pp. 3238, (2020) (PubMed).

Sharif, Wang, Wang, Magupalli, Andreeva, Qiao, Hauenstein, Wu, Núñez, Mao, Wu: "Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome." in: Nature, Vol. 570, Issue 7761, pp. 338-343, (2020) (PubMed).

Xue, Xi, Liu, Guo, Zhang, Zhang, Li, Yang, Zhou, Yang, Zhang, Zhang, Gu, Yang, Da, Yao, Duo, Zhang: "miR-21 promotes NLRP3 inflammasome activation to mediate pyroptosis and endotoxic shock." in: Cell death & disease, Vol. 10, Issue 6, pp. 461, (2020) (PubMed).

Huang, Feng, Jiang, Li, Xiang, Guo, Yang, Fei, Guo, Zheng, Wu, Chen: "VSIG4 mediates transcriptional inhibition of Nlrp3 and Il-1β in macrophages." in: Science advances, Vol. 5, Issue 1, pp. eaau7426, (2020) (PubMed).

Liu, Lu, Chen, Muthuraj, Li, Pattabiraman, Zempleni, Kachman, Natarajan, Yu: "Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice." in: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).

Deng, Guo, Tam, Johnson, Brickey, New, Lenox, Shi, Golenbock, Koller, McKinnon, Beutler, Ting: "Platelet-activating factor (PAF) mediates NLRP3-NEK7 inflammasome induction independently of PAFR." in: The Journal of experimental medicine, Vol. 216, Issue 12, pp. 2838-2853, (2020) (PubMed).

Sanchez-Lopez, Zhong, Stubelius, Sweeney, Booshehri, Antonucci, Liu-Bryan, Lodi, Terkeltaub, Lacal, Murphy, Hoffman, Tiziani, Guma, Karin: "Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production." in: Cell metabolism, Vol. 29, Issue 6, pp. 1350-1362.e7, (2020) (PubMed).

Kiyohara, Sujino, Teratani, Miyamoto, Arai, Nomura, Harada, Aoki, Koda, Mikami, Mizuno, Naganuma, Hisamatsu, Kanai: "Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells." in: Cellular and molecular gastroenterology and hepatology, Vol. 7, Issue 1, pp. 135-156, (2019) (PubMed).

Hafner-Bratkovič, Sušjan, Lainšček, Tapia-Abellán, Cerović, Kadunc, Angosto-Bazarra, Pelegrin, Jerala: "NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway." in: Nature communications, Vol. 9, Issue 1, pp. 5182, (2019) (PubMed).

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Target

NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name

NLRP3/NALP3 (NLRP3 Products)

Synonyms

CIAS1 antibody, Nalp3 antibody, avp antibody, cias1 antibody, nalp3 antibody, pypaf1 antibody, agtavprl antibody, AGTAVPRL antibody, AII antibody, AVP antibody, C1orf7 antibody, CLR1.1 antibody, FCAS antibody, FCU antibody, MWS antibody, NALP3 antibody, PYPAF1 antibody, AII/AVP antibody, Cias1 antibody, Mmig1 antibody, Pypaf1 antibody, NLR family pyrin domain containing-like antibody, NLR family pyrin domain containing 3 antibody, NLR family, pyrin domain containing 3 L homeolog antibody, NLR family, pyrin domain containing 3 antibody, NLRPL antibody, NLRP3 antibody, nlrp3.L antibody, Nlrp3 antibody

Background

The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

UniProt

Q8R4B8

Pathways

Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome