HLA Class I Heavy Chain antibody
Reactivity: Human
ELISA, WB, FACS, IP, EM, ICC, IHC (fro), IHC (p)
Host: Mouse
Monoclonal
HC10
unconjugated
(10 references)-
- Target
- HLA Class I Heavy Chain
- Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
-
Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Electron Microscopy (EM), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- The mouse monoclonal antibody HC10 recognizes HLA class I heavy chains. HC10 reacts mostly with HLA-B and HLA-C heavy chains and some HLA-A (HLA-A10, HLA-A28, HLA-A29, HLA-A30, HLA-A31, HLA-A32, HLA-A33).
- Immunogen
- HC10 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
- Clone
- HC10
- Isotype
- IgG2a
-
anti-HLA Class I Heavy Chain antibody
Reactivity: Human ELISA, WB, FACS, IP, EM, ICC, IHC (fro), IHC (p) Host: Mouse Monoclonal HCA2 unconjugated
-
- Application Notes
- HC10 was raised against free class I heavy chains of HLA antigens to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of formalin-fixed, paraffin-embedded sections, and cryo-immuno-electron microscopy. HC10 indeed retains strong reactivity with free class I heavy chains in Western blots. HC10 also produces strong reactivity in immuno-electron microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigen and can be used to specifically block free heavy chains (FHC), See reference 6. Optimal antibody dilutions for the different applications should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
- Restrictions
- For Research Use only
-
- Buffer
- Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C, or in small aliquots at -20°C.
-
-
: "Mutations in the IFNγ-JAK-STAT pathway causing resistance to immune checkpoint inhibitors in melanoma increase sensitivity to oncolytic virus treatment." in: Clinical cancer research : an official journal of the American Association for Cancer Research, (2021) (PubMed).
: "Analysis of the Tumor Reactivity of Tumor-Infiltrating Lymphocytes in a Metastatic Melanoma Lesion that Lost Major Histocompatibility Complex Class I Expression after Anti-PD-1 Therapy." in: The Journal of investigative dermatology, Vol. 139, Issue 7, pp. 1490-1496, (2020) (PubMed).
: "Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids. ..." in: Cell, Vol. 174, Issue 6, pp. 1586-1598.e12, (2019) (PubMed).
: "Significance of tumor-infiltrating lymphocytes before and after neoadjuvant therapy for rectal cancer." in: Cancer science, Vol. 109, Issue 4, pp. 966-979, (2018) (PubMed).
: "HLA Class I Antigen Expression in Conjunctival Melanoma Is Not Associated With PD-L1/PD-1 Status." in: Investigative ophthalmology & visual science, Vol. 59, Issue 2, pp. 1005-1015, (2018) (PubMed).
: "Increased PD-L1 and T-cell infiltration in the presence of HLA class I expression in metastatic high-grade osteosarcoma: a rationale for T-cell-based immunotherapy." in: Cancer immunology, immunotherapy : CII, Vol. 66, Issue 1, pp. 119-128, (2017) (PubMed).
: "Many de novo donor-specific antibodies recognize β2 -microglobulin-free, but not intact HLA heterodimers." in: HLA, Vol. 87, Issue 5, pp. 356-66, (2017) (PubMed).
: "Monitoring native HLA-I trimer specific antibodies in Luminex multiplex single antigen bead assay: Evaluation of beadsets from different manufacturers." in: Journal of immunological methods, Vol. 450, pp. 73-80, (2017) (PubMed).
: "Conformational Variants of the Individual HLA-I Antigens on Luminex Single Antigen Beads Used in Monitoring HLA Antibodies: Problems and Solutions." in: Transplantation, Vol. 101, Issue 4, pp. 764-777, (2017) (PubMed).
: "HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130811, (2016) (PubMed).
-
: "Mutations in the IFNγ-JAK-STAT pathway causing resistance to immune checkpoint inhibitors in melanoma increase sensitivity to oncolytic virus treatment." in: Clinical cancer research : an official journal of the American Association for Cancer Research, (2021) (PubMed).
-
- Target
- HLA Class I Heavy Chain
- Background
- The HLA class I gene family is composed of a group of genes whose products encode cell surface glycoproteins of MW 40-45 kDa, associated non-covalently with the beta-2-microglobulin light chain. They include the three polymorphic molecules HLA-A, -B, and -C, which are ubiquitously expressed and which are able to present intracellular peptides to cytotoxic T cells. Three additional class I genes are known, commonly referred to as non-classical or class Ib genes, all highly homologous to the other class I genes and all of which associate with beta-2-microglobulin light chain. In humans, each of the class Ib genes appears to exhibit a distinct pattern of expression in developing and adult tissues. HLA-E transcripts are distributed widely in adult tissues and have also been found in the placenta and fetal liver. In the adult, the presence of HLA-F has been shown in skin, resting T cells, and B cells, whereas its expression during development has been reported in fetal liver and at low levels in placenta and extra-placental tissues. HLA-G was originally thought to be expressed only in certain populations of placental trophoblasts, but low levels have also been found in a variety of human tissues. Recently it was shown that HLA class I expression in breast cancer cells can have a predictive value for chemotherapy response.
-
You are here:
